vernonioside A1
compound / compound-vernonioside-a1
ベルノニオシドA1
Vernonioside A1 is a steroid glycoside isolated from the leaves of Vernonia amygdalina. The minimum amount causing bitter taste in a sensory test using a paper disc is 7 µg (Ohigashi et al. 1991).
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Reading the bitterness
Vernonioside A1 is a steroid glycoside isolated from the dried leaves of Vernonia amygdalina. The starting material for isolation was 800 g of dried leaves, obtained through fractionation and purification. The sensory indicator was assessed by the paper disc method (a tentative test in which a sample is applied to a paper disc 1 cm² in area with a thickness of 0.2 mm, and placed in the mouth), and this compound produced a bitter taste at a small amount of 7 µg, with a strength comparable to that of quinine sulfate.
Vernonioside B1, a structurally related glycoside differing from vernonioside A1 only in the presence or absence of a hydroxyl group at the C(16) position, showed no bitter taste in the same paper disc test even when 200 µg was used. From this comparison, the authors suggest the possibility that hydroxylation at C(16) is involved in the expression of bitterness; however, this remains the authors' own speculation and is not a direct experimental verification including B1 and other related compounds.
Sources for this overview
Checked for this overview: The 7 µg minimum amount for bitter taste determined by paper disc sensory test was used. / Structural comparison between A1 and B1 and the suggestion of C(16) hydroxylation were used.
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ndolé →contains→ vernonioside A1
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Composition and content (1)
Scope: Layer: Food (isolated compound from plant tissue). The compound was obtained from 800 g of dried leaves through fractionation using Amberlite XAD-2, silica gel, and ODS, followed by HPLC purification, and evaluated by a tentative sensory test using a paper disc (1 cm², thickness 0.2 mm).
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dish-ndole —contains→ compound-vernonioside-a1Mechanism and receptors (1)
Scope: Layer: Solution. Structural comparison of vernonioside A1 and vernonioside B1 (both C29 stigmastane-type steroid glycosides) isolated from dried leaves of Vernonia amygdalina, and sensory test using a paper disc (1 cm², thickness 0.2 mm).
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compound-vernonioside-a1 —differs_from→ "When vernonioside A1 is compared with vernonioside B1, a structurally related glycoside differing only in the presence or absence of a hydroxyl group at the C(16) position, B1 showed no bitter taste in the same paper disc sensory test even at 200 µg. From this structural difference, the authors suggest the possibility that hydroxylation at C(16) is involved in the expression of bitterness."