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spinach

ingredient / ingredient-spinach

ほうれん草 / Spinacia oleracea

Spinach is a leafy vegetable containing a relatively high amount of oxalic acid. Its unpleasant aftertaste has traditionally been attributed to oxalic acid (egumi), but oxalate taste and bitterness have been reported to separate into different fractions by solid-phase extraction.

On this page (6 sections)
  1. Overview
  2. Sources for this overview (2)
  3. Topic hubs
  4. Other names
  5. Related entries (10)
  6. Sensory (7)
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Reading the bitterness

The bitterness of raw leaves when eaten differed by sample origin. When a trained panel of 6 evaluated about 10 g of unseasoned raw leaf 3 times each on a 15-point scale, the mean ± standard deviation of bitterness was 3.0±0.56 for the open-field product and 2.4±0.50 for the commercial product, with the open-field product significantly higher. The tunnel-covered product was 2.8±0.63, intermediate between the two. Because the commercial product's cultivar, growing method, and days since harvest were unknown, the difference cannot be determined to be due to growing method alone.

In another test that examined salt-boiling, spinach was boiled for 1 minute in distilled water or 1-3% saline at 10 times its weight, then soaked in changing water for a total of 5 minutes. When 10-21 women aged 19 to their 40s answered on a 5-point scale, there was a tendency for egumi to be felt less as the salt concentration increased, but there was no significant difference among the concentration conditions. What was scored here was egumi, not bitterness itself.

In a test from the same paper that varied the type of salt, samples boiled with 3% refined salt or 3% table salt were significantly less egumi than samples boiled with water alone (p<0.01). 「天塩」 (Amashio) salt, however, showed no difference from water-boiling. It cannot be said that any salt gives the same result, and egumi cannot be read as a measurement of bitterness.

Sources for this overview

  1. Reducing food losses and improving the quality of locally produced spinach

    Konstantinos Batziakas / 2019 / thesis / full text checked

    Checked for this overview: Used for the trained panel of 6, about 10 g of unseasoned raw leaf, the 15-point scale, 3 replicates, mean ± standard deviation, the overall test and letter groupings, and the unknown conditions of the commercial product.

  2. 食塩添加ゆでホウレンソウのえぐ味の感じ方とシュウ酸および無機成分含量との関連

    和泉眞喜子 / 2005 / journal article / full text checked

    Checked for this overview: Used for the 10-times boiling water, 1-3% saline, 1-minute boil, total 5-minute soak, 10-21 women, the 5-point egumi evaluation, and the absence of significant difference among concentrations. / Used for the p<0.01 between 3% refined salt/table salt and water-boiling, the 「天塩」 (Amashio) condition, and the limitation of the egumi scale.

Other names: ホウレンソウ (ja)

Structured claims — open a section to read each claim's confidence, evidence and limits

Sensory (7)

spinach — perceived bitterness is suppressed by: Adding a small amount of sugar (0-1.8%, 4 levels) or salt (0-0.2%) to spinach puree lowered bitterness intensity. Other sensory attributes such as texture and aroma did not change.preliminary

Scope: Layer: food. Green-vegetable purees of broccoli, spinach, and kale, evaluated by a trained panel of 9 adults (Experiment 1).

Note: Based on a summary reached via a search engine (the full original text has not been verified). The same trend is reported for all 3 vegetables, and the figures limited to spinach alone cannot be separated out from the summary.

A trained panel of 9 adults evaluated broccoli, spinach, and kale purees with sugar (0/0.6/1.2/1.8%) or salt (0/0.2%) added, and reported that bitterness decreased in all 3 vegetables while other sensory attributes did not change.
Limitations: The full original text has not been obtained; based on a search-engine summary. Figures for spinach alone cannot be separated out from the summary.
Source: Mary Poppins was right: Adding small amounts of sugar or salt reduces the bitterness of vegetables (Bakke AJ; Stubbs CA; McDowell EH; Moding KJ; Johnson SL; Hayes JE, 2018) · abstract checked
Show dataingredient-spinach —has_perceived_bitterness_suppressed_by→ "Adding a small amount of sugar (0-1.8%, 4 levels) or salt (0-0.2%) to spinach puree lowered bitterness intensity. Other sensory attributes such as texture and aroma did not change."
claim: clm-w68a-spinach-sugar-salt-reduces-bitterness / type: sensory / updated: 2026-09-06
spinach — perceived bitterness is suppressed by: Spinach purée with 2% added sugar was chosen as less bitter in a forced-choice task against purée without additives.preliminary

Scope: Layer: Food. Vegetable purées of spinach, kale, and broccoli; a forced-choice task with 84 adults (Experiment 2).

Note: Based on a summary obtained through a search engine (the full original was not confirmed).

It describes preparing each vegetable purée with 0% or 2% sugar and evaluating bitterness in a forced-choice task with 84 adults.
Limitations: The full original was not obtained. The direction of the spinach-only result is not stated in the summary, but it is recorded as a consistent trend together with Experiments 1 and 3.
Source: Mary Poppins was right: Adding small amounts of sugar or salt reduces the bitterness of vegetables (Bakke AJ; Stubbs CA; McDowell EH; Moding KJ; Johnson SL; Hayes JE, 2018) · abstract checked
Show dataingredient-spinach —has_perceived_bitterness_suppressed_by→ "Spinach purée with 2% added sugar was chosen as less bitter in a forced-choice task against purée without additives."
claim: clm-w68a-spinach-sugar-2pct-forced-choice-bitterness / type: sensory / updated: 2026-09-06
spinach — sensory acceptability was increased by: Adding 1% or 2% sugar increased liking for spinach purée in a 9-point hedonic evaluation by 99 adult consumers.preliminary

Scope: Layer: Food. Experiment 3 (9-point hedonic evaluation, 99 adults).

Note: Based on a summary obtained through a search engine (the full original was not confirmed). This concerns an increase in liking scores, not an evaluation of bitterness intensity itself.

Each vegetable purée was prepared with 0/1/2% sugar and evaluated by 99 adults on a 9-point hedonic scale. It describes increased liking among adult consumers with added sugar.
Limitations: The full original was not obtained. These are liking scores, not direct ratings of bitterness intensity.
Source: Mary Poppins was right: Adding small amounts of sugar or salt reduces the bitterness of vegetables (Bakke AJ; Stubbs CA; McDowell EH; Moding KJ; Johnson SL; Hayes JE, 2018) · abstract checked
Show dataingredient-spinach —has_sensory_acceptability_increased_by→ "Adding 1% or 2% sugar increased liking for spinach purée in a 9-point hedonic evaluation by 99 adult consumers."
claim: clm-w68a-spinach-sugar-increases-liking / type: sensory / updated: 2026-09-06
spinach — perceived bitterness is affected by: When spinach-leaf-blade extract was diluted 20-fold, bitterness became markedly weaker than in fresh-leaf extract, while oxalate taste remained relatively strong; the two showed contrasting dilution responses.supported

Scope: Layer: Food. Sensory evaluation of spinach-leaf-blade extract (undiluted and 20-fold diluted).

「20倍希釈液においても、比較的強く感じられるシュウ酸味と、希釈によって刺激が弱まる苦味は対照的であり、ホウレンソウの苦味については、濃度依存的に味細胞を刺激する苦味成分によるものと推定された。」 (in translation: "Even in the 20-fold diluted solution, the relatively strongly perceived oxalate taste contrasts with bitterness whose stimulus weakens with dilution; spinach bitterness was inferred to be caused by bitter components that stimulate taste cells in a concentration-dependent manner.")
Limitations: A quantitative dose-response curve for dilution factor and bitterness intensity could not be confirmed from the readable part of the text; the account remains a qualitative contrast.
Source: ホウレンソウのえぐ味はシュウ酸に由来するか (堀江秀樹; 伊藤秀和, 2006) · full text checked
Show dataingredient-spinach —has_perceived_bitterness_affected_by→ "When spinach-leaf-blade extract was diluted 20-fold, bitterness became markedly weaker than in fresh-leaf extract, while oxalate taste remained relatively strong; the two showed contrasting dilution responses."
claim: clm-w72b1-spinach-bitterness-dilution-response / type: sensory / updated: 2026-09-08
spinach shows the sensory response In a comparison of boiling for 1 minute in distilled water or 1-3% saline followed by a 5-minute water soak, there was a tendency for egumi to be felt less as the salt concentration increased, but there was no significant difference among the concentration conditionssupported

Scope: The cultivar Sunlight, boiling water at 10 times the sample weight, a panel aged 19 to their 40s including female students and staff, a 5-point evaluation

Note: The item evaluated was egumi, not a direct evaluation of bitterness. The 1-3% concentration difference is not treated as a significant effect.

There was no significant difference between water-boiling and 1%, 2%, 3% saline boiling in color, flavor, texture, egumi, or overall evaluation. Egumi showed a tendency to be felt less as the salt concentration increased.
Limitations: The exact number of participants in each individual test cannot be separated out from the text. There was no significant difference among the salt concentration conditions. This is an egumi scale, and it does not independently measure bitterness intensity.
Show dataingredient-spinach —has_sensory_response→ "In a comparison of boiling for 1 minute in distilled water or 1-3% saline followed by a 5-minute water soak, there was a tendency for egumi to be felt less as the salt concentration increased, but there was no significant difference among the concentration conditions"
claim: clm-spinach-salt-boil-acridity-concentration / type: sensory / updated: 2026-09-16
spinach shows the sensory response Samples boiled with 3% refined salt or 3% table salt were significantly less egumi than samples boiled with distilled water alone (p<0.01)supported

Scope: The cultivar Tonic, boiling water at 10 times the sample weight, boiled for 1 minute and soaked in water for 5 minutes, a 5-point sensory evaluation of 4 salt types and distilled water

Note: The 「天塩」 (Amashio) condition showed no significant difference from the distilled-water condition, and this is not generalized to all salts. The item evaluated is egumi.

Boiling with 3% refined salt and boiling with 3% table salt produced significantly less egumi than boiling with water (p<0.01). Boiling with 「天塩」 (Amashio) salt showed no significant difference from boiling with water.
Limitations: Composition differs by salt type, and this is a separate test from the concentration experiment that used the cultivar Sunlight. This is an egumi scale, and it does not independently measure bitterness intensity.
Show dataingredient-spinach —has_sensory_response→ "Samples boiled with 3% refined salt or 3% table salt were significantly less egumi than samples boiled with distilled water alone (p<0.01)"
claim: clm-spinach-salt-type-acridity / type: sensory / updated: 2026-09-16
spinach — perceived bitterness is affected by: In a descriptive evaluation of 3 raw-leaf samples, the bitterness of the open-field product was significantly stronger than the commercial product, and the tunnel-covered product was intermediate, with no significant difference from eithersupported

Scope: Chapter 4 of a doctoral dissertation, a trained panel of 6, about 10 g of unseasoned raw leaf, a 15-point intensity scale, 3 replicates per sample

Note: The open-field product and the tunnel-covered product were both the cultivar Corvair, but the commercial product's cultivar, growing method, and the period from harvest to testing were unknown, so this is not treated as an effect of growing method alone.

On the 15-point scale, bitterness as mean ± standard deviation was 2.8±0.63 for the tunnel-covered product, 3.0±0.56 for the open-field product, and 2.4±0.50 for the commercial product, with an overall test across the 3 samples of p=0.010. The letter groupings from Fisher's protected LSD (p≤0.05) were a for the open-field product, b for the commercial product, and ab for the tunnel-covered product.
Limitations: p=0.010 is the overall test across the 3 samples, not a pairwise p-value for open-field versus commercial. This is a repeated evaluation by a 6-person panel, not treated as a number of independent growing samples. The commercial product's cultivar, growing method, harvest date, and distribution period are unknown. The dissertation chapter was checked in full, but the published paper PDF with the same title was not obtained, so an exact match between versions has not been confirmed.
Source: Reducing food losses and improving the quality of locally produced spinach (Konstantinos Batziakas, 2019) · full text checked
Show dataingredient-spinach —has_perceived_bitterness_affected_by→ "In a descriptive evaluation of 3 raw-leaf samples, the bitterness of the open-field product was significantly stronger than the commercial product, and the tunnel-covered product was intermediate, with no significant difference from either"
claim: clm-spinach-growing-source-bitterness / type: sensory / updated: 2026-09-16

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